Jackson will start again, this time leading off against the Padres. He was 2 for 4 with a walk and a run scored in his debut on Sunday.
Cubs manager Dale Sveum was happy to welcome the young prospects into the fold on Sunday.
"It's nice to get guys in the lineup with fast-twitch fibers and athletes and guys that are going to develop now at the big-league level to see what we've got and move forward," Sveum said. "These guys have got bat speed, they've got hand speed. These are the things you want to see at the big-league level and now they develop."
Sveum moved second baseman Darwin Barney up to the two-hole up from his usual seventh spot, and Starlin Castro will bat fifth for the second straight game.
Here is the full Cubs lineup against the Padres:
1. Jackson CF
2. Barney 2B
3. Anthony Rizzo 1B
4. Alfonso Soriano LF
5. Castro SS
6. Welington Castillo C
7. Vitters 3B
8. Joe Mather RF
9. Travis Wood P